Week 1: Enzymes, enzymes as catalyst, general characteristics, nomenclature and nature of enzymes
Week 2: Structural organization; active site, nature of active site, enzyme
Week 3: Isolation, purification and characterisation of enzymes
Week 4: Measurement of enzyme activity
Week 5-6: Mechanisms of enzyme catalyzed reactions. Effects of temperature,
pH, ions and inhibitors on enzyme catalysed reactions.
Week 7: MichaelisMenton equation allosteric/regulatory enzymes.
Week 8: Estimation of kinetic parameters- enzyme activities, Km, Vmax, Ki etc.
Zymogen activation, digestive enzymes…